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Analysis of institutional authors

Valverde-Pozo, JavierAuthor

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June 7, 2024
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Article

New ICT-Based Ratiometric Two-Photon near Infrared Probe for Imaging Tyrosinase in Living Cells, Tissues, and Whole Organisms

Publicated to: Chemosensors (Basel). 11 (2): 145- - 2023-02-01 11(2), DOI: 10.3390/chemosensors11020145

Authors: Valverde-Pozo, Javier; Paredes, Jose Manuel; Garcia-Rubino, Maria Eugenia; Widmann, Thomas J; Grinan-Lison, Carmen; Lobon-Moles, Silvia; Marchal, Juan Antonio; Alvarez-Pez, Jose Maria; Talavera, Eva Maria

Affiliations

Complejo Hosp Jaen, UGC Oncol Med, Jaen 23007, Spain - Author
Inst Invest Biosanit Ibs Granada, Granada 18012, Spain - Author
Univ Granada, Biopathol & Regenerat Med Inst IBIMER, Ctr Biomed Res CIBM, Granada 18100, Spain - Author
Univ Granada, Ctr Genom & Oncol Res, GENYO, Pfizer,Andalusian Reg Govt, Granada 18016, Spain - Author
Univ Granada, Fac Med, Dept Human Anat & Embryol, Granada 18016, Spain - Author
Univ Granada, Fac Pharm, Unidad Excelencia Quim Aplicada Biomed & Medioambi, Dept Phys Chem,Nanoscopy UGR Lab, Granada 18071, Spain - Author
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Abstract

Melanoma is a type of highly malignant and metastatic skin cancer. In situ molecular imaging of endogenous levels of the melanoma biomarker tyrosinase (TYR) may decrease the likelihood of mortality. In this study, we proposed the weakly fluorescent probe 1-(4-(2-(4-(dicyanomethylene)-4H-chromen-2-yl)vinyl)phenyl)-3-(4-hydroxybenzyl)urea (DCM-HBU), which releases a strong red-shifted fluorescent signal after a TYR-mediated oxidation followed by hydrolysis of the urea linkage. The large Stokes shift of the dye is owed to the recovery of the intramolecular charge transfer (ICT) effect. The resulting probe derivate shows a highly ratiometric fluorescence output. Furthermore, the simultaneous excitation by two near-infrared (NIR) photons of the released derivative of dicyanomethylene-4H-pyran (DCM-NH2) fluorophore could avoid the usual drawbacks, such as cellular absorption, autofluorescence, and light scattering, due to an usually short wavelength of the excitation light on biological systems, resulting in images with deeper tissue penetration. In addition, the probe is useful for the quantitative sensing of TYR activity in vivo, as demonstrated in zebrafish larvae. This new ratiometric two-photon NIR fluorescent probe is expected to be useful for the accurate detection of TYR in complex biosystems at greater depths than other one-photon excited fluorescent probes.

Keywords

BioimaginBioimagingEmissionFluorescent-probeInhibitionMelanomaMoietNir sensorTwo-photon excitationTyrosinaseZebrafish

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Chemosensors (Basel) due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position 17/76, thus managing to position itself as a Q1 (Primer Cuartil), in the category Instruments & Instrumentation.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-05:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 4.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 4 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.
  • The number of mentions on the social network X (formerly Twitter): 3 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Valverde Pozo, Javier) .

Awards linked to the item

This work has been supported by the Ministerio de Ciencia e Innovacion (MCIN/AEI/10.13039/501100011033/FEDER) under grants PID2020-114256RB-I00, PID2020-113059GB-C21 and RTI2018-101309-B-C22; and by FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades/Proyecto A-FQM-230-UGR20 and by the Consejeria de Economia, Conocimiento, Empresas y Universidad de la Junta de Andalucia (European Regional Development Fund) under grant P18-FR-2470 and by the Chair "Doctors Galera-Requena in cancer stem cell research". J.V.-P. is supported by a FPU fellowship (FPU17/04749). T.J.W. is financed by a senior postdoctoral stipend of the Consejeria de Salud y Familias (CSyF) of the Regional Government of Andalusia, Spain (RH-0019-2020). C.G.-L. acknowedges the posdoctoral fellowship from Plan Andaluz de Investigacion, Desarrollo e Innovacion (PAIDI 2021-FEDER funds-POSTDOC_21_638).