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Analysis of institutional authors

Gradillas, AnaAuthorBarbas, CoralCorresponding Author

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December 24, 2024
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Deeper insights into the stability of oxylipins in human plasma across multiple freeze-thaw cycles and storage conditions

Publicated to: Journal Of Pharmaceutical And Biomedical Analysis. 255 116587- - 2025-03-15 255(), DOI: 10.1016/j.jpba.2024.116587

Authors:

Moran-Garrido, M; Camunas-Alberca, SM; Sáiz, J; Gradillas, A; Taha, AY; Barbas, C
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Affiliations

Univ Calif Davis, Coll Agr & Environm Sci, Dept Food Sci & Technol, Davis, CA 95616 USA - Author
Univ Calif Davis, Ctr Neurosci, One Shields Ave, Davis, CA 95616 USA - Author
Univ Calif Davis, West Coast Metabol Ctr, Genome Ctr, Davis, CA 95616 USA - Author
Univ San Pablo CEU, CEU Univ, Fac Farm, Ctr Metabol & Bioanal CEMBIO,Urbanizac Monteprinci, Boadilla Del Monte 28660, Madrid, Spain - Author
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Abstract

Oxylipins are signaling lipids derived from the oxidation of polyunsaturated fatty acids (PUFAs). In lipidomic studies, human plasma may be subjected to various storage conditions and freeze-thaw cycles, which may impact the analysis of these compounds. In this study, we used liquid chromatography coupled with mass spectrometry (LC-MS) to examine the influence of up to five freeze-thaw cycles (FTCs) on free and total (mostly esterified) oxylipins in human plasma and the influence of temperature and storage duration (4 degrees C for up to 120 h and -20 degrees C and -80 degrees C for 1-98 days) in the presence or absence of butylated hydroxytoluene (BHT) on extracted oxylipins stored in LC-MS amber vials. In fresh plasma subjected to several FTCs, approximately 48 % of the detected free oxylipins were significantly altered by the third cycle, with increases in cytochrome P450 (CYP450) and lipoxygenase (LOX)-derived compounds and reductions in trihydroxylated oxylipins. In contrast, multiple FTCs did not significantly alter esterified oxylipins. At 4 degrees C, the extracted oxylipins did not change significantly for up to 120 h (5 days). Oxylipin levels remained stable for 98 days at -80 degrees C but decreased by 98 days at -20 degrees C. The antioxidant activity of butylated hydroxytoluene (BHT) did not influence oxylipin stability at 4 degrees C for 120 h or at -80 degrees C for 98 days, but it reduced oxylipin degradation at -20 degrees C at 98 days. Conversely, prostaglandin F2 alpha (PGF2 alpha) exhibited substantial increases at -20 degrees C and -80 degrees C, independent of BHT. This study demonstrates that (i) unlike free oxylipins, the esterified oxylipin pool remains stable following repeated FTCs, (ii) extracted oxylipins are stable at 4 degrees C for up to 120 h and at -80 degrees C for up to 98 days, but not at -20 degrees C for 98 days, and (iii) BHT may minimize oxylipin degradation of sample extracts stored at -20 degrees C. This study provides a framework for measuring oxylipins under various freeze-thaw and storage conditions.
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Keywords

Butylated hydroxytolueneChromatography, liquidCytochrome p-450 enzyme systemEsterified oxylipinsFatty acids, unsaturatedFree oxylipinsFreeze-thawFreezingHuman plasmaHumansLc-ms/msLipoxygenaseMass spectrometryOxylipinsRedox lipidomicsStabilityTandem mass spectrometryTemperature

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal Of Pharmaceutical And Biomedical Analysis due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 134/352, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Pharmacology & Pharmacy. Notably, the journal is positioned en el Cuartil Q2 para la agencia Scopus (SJR) en la categoría Pharmaceutical Science.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-01-15:

  • WoS: 2
  • Scopus: 2
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-01-15:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 6 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: United States of America.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Moran-Garrido, Maria) and Last Author (BARBAS ARRIBAS, CORAL).

the author responsible for correspondence tasks has been BARBAS ARRIBAS, CORAL.

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Awards linked to the item

Maria Moran-Garrido and Sandra M. Camunas-Alberca acknowledge their predoctoral fellowship from the Spanish Ministry of Universities with references FPU19/06206 and FPU21/01142 respectively.
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