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Analysis of institutional authors

Bonet, FernandoAuthor

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February 7, 2025
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CCBE1 Is Essential for Epicardial Function during Myocardium Development

Publicated to: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 23 (20): 12642- - 2022-10-01 23(20), DOI: 10.3390/ijms232012642

Authors:

Bonet, Fernando; Anez, Sabrina Brito; Inacio, Jose Manuel; Futschik, Matthias E; Belo, Jose Antonio
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Affiliations

Imperial Coll London, Fac Med, MRC London Inst Med Sci LMS, Hammersmith Hosp Campus,Du Cane Rd, London W12 0NN, England - Author
Univ Cadiz UCA, Sch Med, Med Dept, Cadiz 11003, Spain - Author
Univ Nova Lisboa, NOVA Med Sch, Stem Cells & Dev Lab, iNOVA4Hlth,Fac Ciencias Med, P-1169056 Lisbon, Portugal - Author
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Abstract

The epicardium is a single cell layer of mesothelial cells that plays a critical role during heart development contributing to different cardiac cell types of the developing heart through epithelial-to-mesenchymal transition (EMT). Moreover, the epicardium is a source of secreted growth factors that promote myocardial growth. CCBE1 is a secreted extracellular matrix protein expressed by epicardial cells that is required for the formation of the primitive coronary plexus. However, the role of CCBE1 during epicardial development was still unknown. Here, using a Ccbe1 knockout (KO) mouse model, we observed that loss of CCBE1 leads to congenital heart defects including thinner and hyper-trabeculated ventricular myocardium. In addition, Ccbe1 mutant hearts displayed reduced proliferation of cardiomyocyte and epicardial cells. Epicardial outgrowth culture assay to assess epicardial-derived cells (EPDC) migration showed reduced invasion of the collagen gel by EPDCs in Ccbe1 KO epicardial explants. Ccbe1 KO hearts also displayed fewer nonmyocyte/nonendothelial cells intramyocardially with a reduced proliferation rate. Additionally, RNA-seq data and experimental validation by qRT-PCR showed a marked deregulation of EMT-related genes in developing Ccbe1 mutant hearts. Together, these findings indicate that the myocardium defects in Ccbe1 KO mice arise from disruption of epicardial development and function.
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Keywords

AnimalsCardiomyocyte proliferationCcbe1Cell invasionCollagenCoronary vessel developmentEmtEpicardial derived cellsEpicardiumEpithelial-mesenchymal transitionGrowthHeartHeart developmentInductionMesenchymal transitionMiceMice, knockoutMyocardial growtMyocardial growthMyocardiumOrganogenesisPericardiumReceptoTgf-betaVascular smooth-muscle

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2022, it was in position 66/285, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-05:

  • WoS: 3
  • Scopus: 3
  • Europe PMC: 2
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-05:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 11.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 11 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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    Leadership analysis of institutional authors

    This work has been carried out with international collaboration, specifically with researchers from: Portugal; United Kingdom.

    There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Bonet Martínez, Fernando) .

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    Awards linked to the item

    This work was supported by Fundacao para a Ciencia e a Tecnologia (FCT) grants (PTDC/MEC-CAR/29590/2017) to JA Belo and by the Scientific Employment Stimulus to JMI (Norma Transitoria 8189/2018, FCT), and iNOVA4Health-UIDB/Multi/04462/2013, a program financially supported by Fundacao para a Ciencia e Tecnologia/Ministerio da Educacao e Ciencia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement.
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