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Analysis of institutional authors

Gonzalez-Riano, CarolinaAuthorLeon-Espinosa, GonzaloAuthorGarcia, AntoniaAuthorBarbas, CoralCorresponding Author

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February 16, 2025
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Advanced lipidomics using UHPLC-ESI-QTOF-MS/MS reveals novel lipids in hibernating syrian hamsters

Publicated to: JOURNAL OF CHROMATOGRAPHY A. 1743 465692- - 2025-02-22 1743(), DOI: 10.1016/j.chroma.2025.465692

Authors:

Gonzalez-Riano, C; León-Espinosa, G; Regalado-Reyes, M; García, A; Defelipe, J; Barbas, C
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Affiliations

CSIC, Inst Cajal, Ave Doctor Arce 37, Madrid 28002, Spain - Author
Univ CEU San Pablo, Fac Farm, Campus Monteprincipe, Madrid 28668, Spain - Author
Univ Politecn Madrid, Lab Cajal Circuitos Corticales CTB, Campus Montegancedo, Madrid 28223, Spain - Author
Univ San Pablo CEU, Fac Farm, Ctr Metabol & Bioanal CEMBIO, CEU Univ, Boadilla Del Monte 28660, Spain - Author
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Abstract

Mammalian hibernation offers a unique model for exploring neuroprotective mechanisms relevant to neurodegenerative diseases. In this study, we employed untargeted lipidomics with iterative tandem mass spectrometry (MS/MS) to profile the brain lipidome of Syrian hamsters across different hibernation stages: late torpor, arousal, and euthermia (control). Previously, a lipid species identified as methyl-PA(16:0/0:0) showed a significant increase during torpor, but its precise structure was unresolved due to technological constraints. Leveraging iterative MS/MS and advanced lipid annotation tools (LipidAnnotator and MS-DIAL), we accurately annotated 377 lipid species, including the re-identification of methyl-PA(16:0/0:0) as methylated lysophosphatidic acid (PMeOH 16:0/0:0). This reannotation led to the discovery of two additional lipids during torpor: PMeOH 18:0/ 0:0 and PMeOH 18:1/0:0. Verification involved manual inspection of MS/MS spectra and Kendrick Mass Defect plots. The lipid alterations observed during torpor suggest biochemical adaptations to maintain membrane fluidity and protect against oxidative stress under hypothermic conditions. Elevated levels of PMeOH lipids and their lyso-forms may play roles in cell survival signalling. Additionally, a decrease in phosphatidic acid species and an increase in diacylglycerol species imply a metabolic shift favouring diacylglycerol production, potentially activating protein kinase C signalling pathways. The increased levels of monogalactosyl diglyceride lipids during torpor suggest a role in neuroprotection by enhancing oligodendrocyte function and myelination. Our comprehensive lipidomic profiling provides detailed insights into lipid dynamics associated with hibernation and underscores the potential of advanced MS/MS methodologies in lipidomics for developing therapeutic strategies against neurodegenerative diseases.
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Keywords

AcquisitionAnimalsBrainBrain lipidomeChromatography, high pressure liquidCoveragCricetinaeDdaHibernationLipid metabolismLipidomicsLipidsMaleMesocricetusNovel lipidNovel lipidsSpectrometry, mass, electrospray ionizationTandem mass spectrometryUntargeted lipidomics

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal JOURNAL OF CHROMATOGRAPHY A due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 17/86, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemical Research Methods.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-04:

  • WoS: 2
  • Scopus: 2
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-04:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 7.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 7 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (González Riaño, Carolina) and Last Author (BARBAS ARRIBAS, CORAL).

the author responsible for correspondence tasks has been BARBAS ARRIBAS, CORAL.

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Awards linked to the item

This work was supported by Grant PID2021-122490NB-I00 funded by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe (CGR, AG, and CB) and COST Action 19105-EpiLipidNET (CGR and AG).
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