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Bravo, BeatrizAuthor

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June 4, 2025
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CD271+ stromal cells expand in arthritic synovium and exhibit a proinflammatory phenotype

Publicated to: ARTHRITIS RESEARCH & THERAPY. 18 66- - 2016-03-15 18(), DOI: 10.1186/s13075-016-0966-5

Authors:

Del Rey, Manuel J; Fare, Regina; Usategui, Alicia; Canete, Juan D; Bravo, Beatriz; Galindo, Maria; Criado, Gabriel; Pablos, Jose L
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Affiliations

Hosp 12 Octubre, Serv Cirugia Ortoped & Traumatol, E-28041 Madrid, Spain - Author
Hosp Clin Barcelona, Serv Reumatol, Unitat Artrit, Barcelona, Spain - Author
Inst Invest Biomed August Pi & Sunyer, Barcelona, Spain - Author
Inst Invest Hosp 12 Octubre I 12, Serv Reumatol, Madrid 28041, Spain - Author
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Abstract

Background: CD271(+) stromal cells (SCs) with multipotent stem cell capacity have been identified in synovial tissues, but their functional significance is unknown. We analyzed the distribution of CD271(+) cells in inflammatory synovial tissues as well as their ex vivo immunomodulatory and inflammatory phenotypes. Methods: CD271 expression was analyzed by immunohistochemistry in synovial tissues and by flow cytometry in primary adherent synovial cell cultures from rheumatoid arthritis (RA), osteoarthritis (OA), and non-inflammatory control tissues. Isolation of CD271(+) synovial SCs was carried out by magnetic cell sorting. Allogeneic T-cell/SC cocultures were performed to analyze the regulatory capacity of these cells on T-cell proliferation and cytokine production. The production of inflammatory mediators was analyzed in cultures of sorted CD271(+)/(-) SCs. The capacity of CD271(+)/(-) SCs to induce inflammatory cell recruitment in vivo was evaluated in subcutaneous implants in immunodeficient mice. Results: CD271(+) SC were detected in non-inflammatory as well as in arthritic synovial tissues with a specific perivascular distribution. CD271(+) SC density was increased in RA and OA compared with normal synovial tissues. T-cell proliferation and cytokine synthesis were similarly modified by CD271(+) and CD271(-) SCs. Sorted CD271(+) SCs from OA synovial tissues released significantly more interleukin (IL)-6, matrix metalloproteinase (MMP)-1, and MMP-3 than CD271(-) SCs. In immunodeficient mice, implants of CD271(+) SCs induced significantly higher myeloid cell infiltration than CD271(-) SCs. Conclusions: Our results demonstrate that CD271(+) perivascular SCs expand in RA and OA synovial tissues. CD271(+) cells showed enhanced proinflammatory properties ex vivo and in vivo, whereas immunoregulatory properties were equivalent in CD271(+) and CD271(-) SC.
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Keywords

BoneFibroblastsIl-In-vitroMesenchymal stem cellsMesenchymal stem-cellsNerve growth factor receptoOsteoarthritisRheumatoid arthritisRheumatoid-arthritisStromal cellsSynovial fibroblasts

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal ARTHRITIS RESEARCH & THERAPY due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2016, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Rheumatology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-04:

  • WoS: 15
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-04:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 33.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 33 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Awards linked to the item

We are grateful to Vanessa Miranda for technical assistance. This work was supported by Instituto de Salud Carlos III (FIS12/439, RFTICS RD12/009 RIER, Rio Hortega program [to RF] and Miguel Servet program [to GC]) and was cofinanced by FEDER and Comunidad de Madrid (RAPHYME-CM S2010/BMD2350).
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