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Analysis of institutional authors

Redondo, PAuthor

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March 17, 2016
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Article

The Use of P63 Immunohistochemistry for the Identification of Squamous Cell Carcinoma of the Lung

Publicated to: Plos One. 5 (8): e12209- - 2010-10-20 5(8), DOI: 10.1371/journal.pone.0012209

Authors: Conde, Esther; Angulo, Barbara; Redondo, Pilar; Toldos, Oscar; Garcia-Garcia, Elena; Suarez-Gauthier, Ana; Rubio-Viqueira, Belen; Marron, Carmen; Garcia-Lujan, Ricardo; Sanchez-Cespedes, Montse; Lopez-Encuentra, Angel; Paz-Ares, Luis; Lopez-Rios, Fernando;

Affiliations

Hop Llobregat, Genes & Canc Grp, PEBC, IDIBELL, Barcelona, Spain - Author
Hosp Univ 12 Octubre, Dept Pulm, Madrid, Spain - Author
Hosp Univ Virgen Rocio, Seville, Spain - Author
Inst Biomed Sevilla, Dept Oncol, Seville, Spain - Author
Pathol Thorac Surg & Hosp Univ 12 Octubre, Madrid, Spain - Author
Univ San Pablo, CEU, Dept Oncol, Hosp Univ Madrid Sanchinarro, Madrid, Spain - Author
Univ San Pablo, CEU, Lab Dianas Terapeut, Ctr Integral Oncol Clara Campal,Hosp Univ Madrid, Madrid, Spain - Author
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Abstract

While some targeted agents should not be used in squamous cell carcinomas (SCCs), other agents might preferably target SCCs. In a previous microarray study, one of the top differentially expressed genes between adenocarcinomas (ACs) and SCCs is P63. It is a well-known marker of squamous differentiation, but surprisingly, its expression is not widely used for this purpose. Our goals in this study were (1) to further confirm our microarray data, (2) to analize the value of P63 immunohistochemistry (IHC) in reducing the number of large cell carcinoma (LCC) diagnoses in surgical specimens, and (3) to investigate the potential of P63 IHC to minimize the proportion of "carcinoma NOS (not otherwise specified)" in a prospective series of small tumor samples.With these goals in mind, we studied (1) a tissue-microarray comprising 33 ACs and 99 SCCs on which we performed P63 IHC, (2) a series of 20 surgically resected LCCs studied for P63 and TTF-1 IHC, and (3) a prospective cohort of 66 small thoracic samples, including 32 carcinoma NOS, that were further classified by the result of P63 and TTF-1 IHC.The results in the three independent cohorts were as follows: (1) P63 IHC was differentially expressed in SCCs when compared to ACs (p<0.0001); (2) half of the 20 (50%) LCCs were positive for P63 and were reclassified as SCCs; and (3) all P63 positive cases (34%) were diagnosed as SCCs.P63 IHC is useful for the identification of lung SCCs.

Keywords

AdenocarcinomaAmplificationCancerClassificationGene-expressionOverexpressionPhase-iiiProliferationsTissue microarrayTumors

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Plos One due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2010, it was in position 12/86, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-12-06:

  • WoS: 45
  • Scopus: 49
  • Europe PMC: 32

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-06:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 43 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Conde, E) .

the authors responsible for correspondence tasks have been Conde, E and Garcia-Garcia, E.

Awards linked to the item

This work was partially funded by grants from Fundacion Mutua Madrilena to EC, FLR, and LPA; CIBER Respiratory Disease to ALE (ISCIII-CB06/06); and Red Tematica de Investigacion Cooperativa en Cancer (RTICC) to MSC (RD06/0020/0062). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.