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Analysis of institutional authors

Gonzalez-Riano CAuthorBarbas CAuthor

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March 9, 2019
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Quantitative metabolic profiling of urinary eicosanoids for clinical phenotyping

Publicated to: Journal Of Lipid Research. 60 (6): 1164-1173 - 2019-06-01 60(6), DOI: 10.1194/jlr.d090571

Authors:

Gómez, C; Gonzalez-Riano, C; Barbas, C; Kolmert, J; Ryu, MH; Carlsten, C; Dahlén, SE; Wheelock, CE
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Affiliations

Karolinska Inst, Dept Med Biochem & Biophys, Div Physiol Chem 2, Stockholm, Sweden - Author
Karolinska Inst, Inst Environm Med, Unit Lung & Allergy Res, Stockholm, Sweden - Author
Univ British Columbia, Air Pollut Exposure Lab, Chan Yeung Ctr Occupat & Environm Resp Dis, Dept Med, Vancouver, BC, Canada - Author
Univ CEU San Pablo, Fac Farm, Ctr Metabol & Bioanal CEMBIO, Madrid, Spain - Author
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Abstract

© 2019 American Society for Biochemistry and Molecular Biology Inc.. All rights reserved. The eicosanoids are a family of lipid mediators of pain and inflammation involved in multiple pathologies, including asthma, hypertension, cancer, atherosclerosis, and neurodegenerative diseases. These signaling mediators act locally, but are rapidly metabolized and transported to the systemic circulation as a mixture of primary and secondary metabolites. Accordingly, urine has become a useful readily accessible biofluid for monitoring the endogenous synthesis of these molecules. Herein, we present the validation of a rapid, repeatable, and precise method for the extraction and quantification of 32 eicosanoid urinary metabolites by LC-MS/MS. For 12 out of 17 deconjugated glucuronide eicosanoids, there was no improvement in recovered signal. These metabolites cover the major synthetic pathways, including prostaglandins, leukotrienes, and isoprostanes. The method linearity was >0.99 for all metabolites analyzed, the limit of detection ranged from 0.05.5 ng/ml, and the average extraction recoveries were >90%. All analytes were stable for at least three freeze/thaw cycles. The method was formatted for large-scale analysis of clinical cohorts, and the long-term repeatability was demonstrated over 15 months of acquisition, evidencing high precision (CV <15%, except for tetranorPGEM and 2,3-dinor-11β-PGF2α, which were <30%). The presented method is suitable for focused mechanistic studies as well as large-scale clinical and epidemiological studies that require repeatable methods capable of producing data that can be concatenated across multiple cohorts.
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Keywords

AllergenAsthmaBiosynthesisChromatography, high pressure liquidCreatinineCysteinyl leukotrienesEicosanoidsGravityHumansInflammationIsoprostanesLeukotriene-e4Lipid mediatorsLiquid chromatography-tandem mass spectrometryMetabolomicsNormalizationProstaglandinsQuantificationSolid phase extractionTandem mass spectrometryThromboxanesUrinary metabolites

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal Of Lipid Research due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2019, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Endocrinology. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations from Scopus Elsevier, it yields a value for the Field-Weighted Citation Impact from the Scopus agency: 1.04, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-01-14, the following number of citations:

  • WoS: 27
  • Scopus: 29
  • Europe PMC: 12
  • Google Scholar: 25
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-01-14:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 58.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 58 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 9.
  • The number of mentions on the social network X (formerly Twitter): 14 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Canada; Sweden.

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Awards linked to the item

This work was supported by Swedish Heart-Lung Foundation Grants 20170603 and 20170734, Swedish Research Council Grant 2016-02798, AllerGen National Center of Excellence Grant GxE4, Stockholm County Council Research Funds (ALF), and the Centre for Allergy Research Highlights Asthma Markers of Phenotype (ChAMP) consortium, which is funded by the Swedish Foundation for Strategic Research, the Karolinska Institutet, AstraZeneca Canada and Science for Life Laboratory Joint Research Collaboration, and the Vardal Foundation. C.E.W. was supported by Swedish Heart-Lung Foundation Grant 20180290. C.C. was supported by the Canada Research Chairs program and the AstraZeneca Canada Chair in Occupational and Environmental Lung Disease.
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