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Analysis of institutional authors

Iturrieta-Zuazo, IgnacioAuthor

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February 3, 2015
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A New Antigen Retrieval Technique for Human Brain Tissue

Publicated to: Plos One. 3 (10): e3378-e3378 - 2008-10-13 3(10), DOI: 10.1371/journal.pone.0003378

Authors: Prensa Sepulveda, Lucia; Alelu-Paz, Raul; Iturrieta-Zuazo, Ignacio; Byne, William; Haroutunian, Vahram; Garcia-Villanueva, Mercedes; Rabano, Alberto; Garcia-Amado, Maria; Manuel Gimenez-Amaya, Jose

Affiliations

Alcorcón Hospital Foundation - Author
Clínica Universitaria de Navarra - Author
Hospital Ramón y Cajal - Author
Icahn School of Medicine at Mount Sinai - Author
Universidad Autonoma de Madrid, Facultad de Medicina - Author
VA Medical Center - Author
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Abstract

Immunohistochemical staining of tissues is a powerful tool used to delineate the presence or absence of an antigen. During the last 30 years, antigen visualization in human brain tissue has been significantly limited by the masking effect of fixatives. In the present study, we have used a new method for antigen retrieval in formalin-fixed human brain tissue and examined the effectiveness of this protocol to reveal masked antigens in tissues with both short and long formalin fixation times. This new method, which is based on the use of citraconic acid, has not been previously utilized in brain tissue although it has been employed in various other tissues such as tonsil, ovary, skin, lymph node, stomach, breast, colon, lung and thymus. Thus, we reported here a novel method to carry out immunohistochemical studies in free-floating human brain sections. Since fixation of brain tissue specimens in formaldehyde is a commonly method used in brain banks, this new antigen retrieval method could facilitate immunohistochemical studies of brains with prolonged formalin fixation times.

Keywords

AntigensBrain chemistryCitraconic acidFemaleFixativesFormaldehydeFumaratesHumansImmunohistochemistryMaleMaleatesMethodsTissue fixation

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Plos One due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2008, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Medicine (Miscellaneous). Notably, the journal is positioned above the 90th percentile.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-12-13:

  • Open Alex: 22
  • WoS: 15
  • Scopus: 18
  • Europe PMC: 14

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-13:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 76.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 76 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 5.

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: San Francisco; United States of America.