Indexat a

Llicència i ús

Citacions

Altmetrics

Grant support

This research was funded by Universidad San Pablo-CEU, CEU Universities grant number MGI21EAA.

Anàlisi d'autories institucional

Compartir

Effect of a Gluten-Free Diet on the Intestinal Microbiota of Women with Celiac Disease

Publicat a:Antibiotics. 14 (8): 785- - 2025-08-02 14(8), DOI: 10.3390/antibiotics14080785

Autors: Serrano, M Mar Morcillo; Reche-Sainz, Paloma; Gonzalez-Reguero, Daniel; Robas-Mora, Marina; de la Iglesia, Rocio; Ubeda, Natalia; Alonso-Aperte, Elena; Arranz-Herrero, Javier; Jimenez-Gomez, Pedro A

Afiliacions

Resum

Background/Objectives: Celiac disease (CD) is an autoimmune disorder characterized by small intestinal enteropathy triggered by gluten ingestion, often associated with gut dysbiosis. The most effective treatment is strict adherence to a gluten-free diet (GFD), which alleviates symptoms. This study uniquely integrates taxonomic, functional, and resistance profiling to evaluate the gut microbiota of women with CD on a GFD. Methods: To evaluate the long-term impact of a GFD, this study analyzed the gut microbiota of 10 women with CD on a GFD for over a year compared to 10 healthy controls with unrestricted diets. Taxonomic diversity (16S rRNA gene sequencing and the analysis of alpha and beta-diversity), metabolic functionality (Biolog EcoPlates (R)), and antibiotic resistance profiles (Cenoantibiogram) were assessed. Results: Metagenomic analysis revealed no significant differences in taxonomic diversity but highlighted variations in the abundance of specific bacterial genera. Women with CD showed increased proportions of Bacteroides, Streptococcus, and Clostridium, associated with inflammation, but also elevated levels of beneficial genera such as Roseburia, Oxalobacter, and Paraprevotella. Despite no significant differences in metabolic diversity, higher minimum inhibitory concentrations (MICs) in women in the healthy control group suggest that dietary substrates in unrestricted diets may promote the proliferation of fast-growing bacteria capable of rapidly developing and disseminating antibiotic resistance mechanisms. Conclusions: These findings indicate that prolonged adherence to a GFD in CD supports remission of gut dysbiosis, enhances microbiota functionality, and may reduce the risk of antibiotic resistance, emphasizing the importance of dietary management in CD.

Paraules clau